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Enzyme-linked immunosorbent assay (ELISA) is to bind soluble antigens or antibodies to solid-phase carriers such as polystyrene and use the characteristics of specific bonding between antigens and antibodies to qualitatively and quantitatively analyze the samples. Methods of quantitative detection.


ELISA in Virus Detection

ELISA mainly realizes virus detection by qualitative or quantitative detection of viral antigens or antibodies. Commonly used ELISA can be divided into the following five categories, including direct ELISA, indirect ELISA, sandwich ELISA, competitive ELISA and competitive inhibition ELISA. ELISA can be used to measure antigens as well as antibodies. There are three necessary reagents in this assay: a) a solid-phase antigen or antibody; b) an enzyme-labeled antigen or antibody; c) a substrate for enzyme action.

Coating Antigen for ELISA

ELISA coating antigens are mainly divided into three categories, natural antigens, synthetic polypeptide antigens and recombinant antigens.

  • Native Antigen as ELISA Coating Antigen
    Natural antigens can be taken from animal tissues, microbial cultures, etc., and are proteins, polysaccharides and binding proteins that exist in nature, such as microbial antigens such as viruses and bacteria, which must be extracted and purified before they can be used for coating.
  • Synthetic Polypeptide as ELISA Coating Antigen
    Synthetic polypeptide antigens use synthetic polypeptides as ELISA coating antigens, which have high purity and specificity, but are difficult to adsorb on solid phase. In addition, when the microbial antigen structure changes, peptide-based ELISA is prone to miss detection.
  • Recombinant Antigen as ELISA Coating Antigen
    Recombinant antigen means that the antigen gene is connected to a vector and then transferred into prokaryotic or eukaryotic cells, and then purified by recombinant expression in vitro. There are few impurities and no infectivity, but the purification technology is difficult. Another feature of recombinant antigens is that some antigenic substances that cannot be separated from natural materials can be prepared by genetic engineering.

VLP as Coating Antigen for ELISA

Virus-like particles (VLPs) belong to the recombinant antigens in the three major categories of ELISA coated antigens, which lack genetic material formed by the self-assembly of viral capsid proteins/structural proteins. Compared with natural viral antigens, VLPs are more suitable as coating antigens to develop detection methods for serological diagnosis due to their high safety and ease of use. VLP-based ELISA is widely used to measure antibodies or neutralizing epitopes with good specificity, reproducibility, and high sensitivity.

How We can Help

As a global leader in the production and application of VLPs from VLPlantTM platform, CD BioSciences leverages its expertise to help our customers develop VLP-based ELISA methods that can be used for clinical disease detection. We are good at tailoring our services to the needs of our clients. Please contact us if you are interested.

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