Lysine-based VLP-Peptide Conjugation Service
As the world's leading customized service provider, CD BioSciences has a sophisticated VLPlantTM platform, advanced equipment and experienced scientists, dedicated to helping our customers connect peptides and VLPs through lysine-based modification. Working closely with our customers and experts, we are happy to meet each of our specific requirements in order to provide the optimal solution.
Lysine-based Modifications of VLPs
This lysine-based chemical conjugation modification has been used to embed transferrin in VLPs, allowing VLPs to cross the blood-brain barrier and opening up a new therapeutic avenue for the treatment of neurological disorders. In addition, peptides, proteins and fluorescent probes can also be embedded in VLPs via the NHS reaction, resulting in cVLPs.
The stoichiometry of peptide display is defined by the quaternary structure, and the self-assembly of the capsid in vivo requires less processing and a more homogeneous final product. Peptides can be linked to VLPs by genetic fusion or chemical conjugation methods, developing cVLPs. The chemical conjugation methods include cysteine modification, lysine modification, acidic amino acid modification and the like.
Peptides and Epitopes
Peptides are small natural biomolecules between amino acids and proteins, formed from amino acid monomers linked by peptide (amide) bonds. Many of the roles played by peptides in immunology are primarily determined by epitopes. Epitopes are the smallest chemical molecules that the immune system can recognize, and major histocompatibility complexes (T-cell epitopes) and antibodies (B-cell epitopes) are central to the adaptive immune response. Peptides mediate adaptive immune responses through the interaction of T-cell epitopes with B-cell epitopes.
Lysine-based VLP-Peptide Conjugation
The classic approach to developing cVLPs by linking peptides to VLPs by chemical coupling methods is based on lysine modifications-using cross-linkers that react with nucleophilic amino acid side chains on VLPs. Because lysine has a primary amine, it can be easily modified. At pH values between 7.2 and 9, surface-exposed lysine residues undergo an ester reaction with n-hydroxysuccinimide (NHS) to form amide bonds (NHS can be released as part of the reaction). Furthermore, this lysine-based chemical conjugation method has been used to intercalate transferrin into VLPs, enabling VLPs to cross the blood-brain barrier, opening new therapeutic avenues for the treatment of neurological diseases.
CD BioSciences has a sophisticated VLPlantTM platform and extensive experience in providing lysine-based VLP-peptide conjugation.
The Workflow of Lysine-based VLP-Peptide Conjugation
Why Choose CD BioSciences
We provide global customers with services of linking peptides and VLPs based on lysine modification for a long time and are good at solving difficulties and challenges encountered in the experimental process.
We maintain close contact with customers, discuss problems encountered in the experimental process in a timely manner, and meet your specific requirements.
We have the ability and confidence to provide you with the best experimental solution to meet your needs for fast and accurate linking of peptides to VLPs.