VLP Purification Service
CD BioSciences is a leading global company with advanced equipment and experienced staff using VLPlantTM platform dedicated to helping our global customers purify VLPs produced in plants to help you develop vaccines, nanomaterials and diagnostics.
Overview of VLPs Purification in Plants
In order to develop virus-like particles constructed from plant-based platforms into vaccines, nanomaterials and diagnostic reagents, the purity, potency and consistency of the virus-like particles are essential to ensure efficacy and safety suitable for clinical use.
- Cell Fragmentation
After production of VLPs by the plant transient expression system, the plants are harvested. The key to the first stage in the purification of VLPs in plants is the ability of the VLPs to be released into the extracellular medium.
In order to reduce the number of steps and costs involved in the purification of VLPs from plants, we need to perform a clarification step to remove plant cell debris and aggregates. We offer a variety of methods that can be used for the clarification step of the VLPs purification process, including cell precipitation, deep filtration and microfiltration tangential flow filtration (TFF).
- Moderate Purification/Concentration
Capture and concentration are a key step in the purification of plant produced VLPs, increasing the concentration of VLPs, significantly reducing the volume of liquid and reducing the volume of liquid used for the next experiment thereby reducing workload.
To separate VLPs from plant cell contamination (e.g., plant cell debris), VLPs are usually captured by ultrafiltration, but also by mixing and dilution using non-linear chromatography, ion exchange chromatography and hydrophobic interaction chromatography.
- Sterile Filtration
In addition, the plant produced VLPs need to be filtered and sterilized through sterile 0.22-micron filters before they can be used in vaccine production, nanomaterial development and diagnostic reagent development.
CD BioSciences offers services covering all experimental components associated with VLP (produced from plants) purification, including steps such as harvesting, clarification, capture and concentration, purification and polishing of VLP.
Workflow of Our Service
After harvesting the plants, chelating agents or protease inhibitors are added and the tissues are broken by grinding, high pressure homogenization, ultrasonication, repeated freezing, thawing and enzymatic treatment.
Our services to clarify VLPs include low-speed centrifugation and membrane filtration methods.
We offer ultracentrifugation, PEG precipitation, membrane concentration, and chromatography methods for the concentration of VLPs.
We usually use affinity or ion-exchange chromatography for high-resolution separation of VLPs. Of course, we also offer ultrafiltration/diafiltration (UF/DF) and size exclusion chromatography (SEC) for polishing VLPs.
To reduce the non-specific adsorption of VLPs on the membrane material, the final product of VLPs is usually subjected to a one-step sterile filtration process like other biopharmaceutical processes.
CD BioSciences is committed to providing our global customers with fast, quality service at competitive prices. If you are interested in our services, please contact us for more details and we will respond promptly.