A. thaliana-based VLP Production Service
CD BioSciences is a leading global company with advanced equipment and experienced staff dedicated to helping our global customers build virus-like particles (VLPs) from Arabidopsis thaliana (A. thaliana) plant to help you develop vaccines, nanomaterials and diagnostic reagents.
Overview of A. thaliana
A. thaliana, also known as mouse-ear mustard and Arabidopsis, is a species of angiosperm native to Eurasia and Africa. Although it has made little direct contribution to agriculture, A. thaliana plays an important role in agricultural science and is a popular model organism in the fields of plant biology and genetics. Currently, A. thaliana is used extensively in the study of plant science, including genetics, evolution, population genetics and plant development.
Advantages of A. thaliana as a Model Organism
A. thaliana has several advantages as a model organism. Firstly, Arabidopsis has a relatively short life cycle, with many strains commonly used in the laboratory going from germination to seed maturity in about six weeks. Secondly the plants are small, making it easy to cultivate in a limited space, and a single plant can produce several thousand seeds. Then A. thaliana has a relatively small genome of about 135 million base pairs (Mbp) and it is the first plant whose genome has been completely sequenced, which can be used as a molecular biology tool to understand plant shape (development and phototropism). In addition, the mechanism of self-pollination in A. thaliana helps genetic experiments. Finally, DNA can be easily transformed into the A. thaliana genome using Agrobacterium rhizogenic.
CD BioSciences offers services covering all experiments related to the construction of VLPs through A. thaliana, including the construction of vectors containing the target gene, plant transient expression systems (Agrobacterium) and harvesting of A. thaliana plants containing VLPs, as well as VLP-Purification.
Workflow of Our Service
Construction of A. thaliana Expression Vectors
It all starts with screening for the target gene or viral code and then using our technology to synthesize genetic instructions that the plant can 'read'. The synthesized target gene is inserted into a bacterium, which then carries the information into A. thaliana plant cell.
Plant Transient Expression System
Transient expression mediated by Agrobacterium allows for faster and more efficient production of VLPs by the plant. The plant expression vector is transformed into the rooting Agrobacterium and the Agrobacterium then infiltrates into the A. thaliana plant. A. thaliana plants are placed in carefully controlled greenhouses to rapidly produce VLPs in large quantities.
Harvest Plants Containing VLPs
Once the plants have finished growing, we harvest the plants and mix the leaves with a solution to extract VLPs. VLPs do not contain genetic material, so they are not infectious. Additionally, plants carry a very low risk of introducing foreign human pathogens.
Purification of VLPs
Our purification services for VLPs constructed from plant platforms include PEG precipitation, ultracentrifugation techniques, and affinity chromatography, to name a few. See the VLP-Purification page for details.
Process of Our Service
We have experienced scientists, technicians and advanced equipment to provide our global customers with a fast, and high-quality service at competitive prices. If you are interested in our services, please contact us for more details, and we will respond promptly.