Biochemical Methods-based VLP Characterization Service
The characterization of purified Virus-like particles (VLPs) constructed by plant platforms is an important research content for the production of VLP-based vaccines. CD BioSciences is a leading global company with advanced equipment and experienced staff dedicated to helping customers with biochemical characterization assays for VLPs.
Biochemical Methods for VLP Characterization
The biochemical characterization of VLPs includes properties such as primary amino acid sequence, molecular weight, isoelectric point and purity. Biochemical methods for the characterization of VLPs include mass spectrum (MS), Sodium Dodecyl Sulfate Poly Acrylamide Gel Electrophoresis (SDS-PAGE) and reversed phase high-performance liquid chromatography (RP-HPLC).
Biochemical methods for characterization of VLPs. (Nooraei S, et al., 2021)
An analytical chemistry technique that combines the physical separation capability of liquid chromatography (LC) with the mass analysis capability of mass spectrometry (MS), and is used in many fields. In general, it is used in complex mixtures of various other compounds, to measure various components and possibly determine their detailed structures.
It consists of MALDI and TOF. The principle of MALDI is to irradiate a co-crystal film formed by a sample and a matrix with a laser, and the matrix absorbs energy from the laser and transmits it to the biomolecules. Protons are transferred to or from biomolecules during ionization. The principle of TOF is that the ions are accelerated to fly through the flight tube under the action of an electric field, and the mass-to-charge ratio (M/Z) of the ions is determined according to the time to reach the detector.
RP-HPLC is a liquid chromatography system consisting of a non-polar stationary phase and a polar mobile phase. The typical stationary phase for RP-HPLC is octadecyl-bonded silica and the typical mobile phases are methanol and acetonitrile. RP-HPLC is a type of chemically bonded phase chromatography using a non-polar bonded stationary phase. In RP-HPLC, the polarity of the bonded stationary phase is less than that of the mobile phase to make it suitable for the separation of non-polar, polar or ionic compounds.
Use the difference in molecular weight of proteins in the sample to separate them in an electrophoresis gel. Since sodium dodecyl sulfate and polyacrylamide respectively eliminate the influence of protein structure and charge during electrophoresis, the migration rate of proteins is only determined by their size.
CD BioSciences offers virtually all biochemical methods used to characterize VLPs, including LC-MS, MALDI-TOF MS, SDS-PAGE and RP-HPLC) for the detection of molecular weight, peptide profile, integrity and purity of VLPs. In addition, we offer various services for the construction of VLPs on plant platforms, including services for the construction of VLPs on various plant platforms, VLP purification, generation of cVLPs, and other methods for the characterization of VLPs (biophysical methods and biological methods). If you are interested in the related content, you can click the link for more information, or of course you can contact us directly and we will reply you promptly.
Why Choose CD BioSciences
Experienced: We have long provided global clients with a variety of biochemical methods to characterize VLPs constructed on plant platforms. In turn, help customers realize the development of vaccines, nanomaterials and diagnostic reagents based on VLPs.
Advanced Equipment: Our laboratory is equipped with the latest and most advanced instrumentation to perform the characterization of VLPs with almost laboratory methods to suit your needs.
Customer-First: From the inquiry to the delivery of the results, we will respond to your messages in a timely manner, keep in touch with you all the time, and provide you with the best experimental plan.
- Nooraei, S.; et al., Virus-like particles: preparation, immunogenicity and their roles as nanovaccines and drug nanocarriers. J Nanobiotechnology. 2021, 25:19-59.