Noncovalent Bonds-based VLP Modification Service

Noncovalent Bonds-based VLP Modification Service

As a leading global provider of VLP services, CD BioSciences' team of experts will provide you with the most professional solutions and sophisticated VLPlant^TM platform to help you modify VLPs through noncovalent bonding methods. To ensure your research progress, our experienced scientists will be happy to meet each of your specific requirements.

Overview of Noncovalent bonds-based VLP Modification

Chemical conjugation methods for modifying VLPs include covalent and non-covalent linkage methods. The most common method of covalent linkage of antigens to VLPs is achieved by using heterobifunctional chemical crosslinkers with amine and sulfhydryl reactive arms. In contrast, non-covalent coupling involves the use of streptavidin as a linker to link biotinylated antigens to VLPs using its specificity and strong interactions.

Streptavidin

Streptavidin (SAv) is a protein purified from the bacteriophage Streptomyces. SAv is widely used to immobilize biotinylated proteins, receptors and DNA. SAv homotetramer has a very high affinity for biotin (i.e., vitamin B7) and is used to immobilize biotinylated proteins, receptors and nucleic acids. As one of the strongest non-covalent interactions in nature, the binding of chain affine to biotin is well tolerated to organic solvents, denaturants (e.g., guanidine hydrochloride), detergents (e.g., SDS), protein hydrolases, and extreme temperatures and pH, and is therefore widely used in molecular biology and bio-nanotechnology.

SAv coverage increasing with supported lipid bilayer biotin content. (Hamming PHE, et al., 2021)

Biotin and Biotinylation

Biotin, so named because its deficiency rarely occurs, is one of the B vitamins, also known as vitamin H, vitamin B7, and coenzyme R. It is an important factor in the fixation of carbon dioxide by living organisms. In biochemistry, biotinylation is the process of covalently attaching biotin to a protein, nucleic acid or other molecule. Because of the small molecular weight of biotin, biotinylation reactions are fast, efficient and not easily interfered with. The biotinylated molecule is able to interact with chain affinity through biotin and is unaffected by high temperature, extreme pH, and proteolysis.

SAv-biotin Technology

The high stability of SAv compared to most other proteins and the availability of biotinylation (for biological, synthetic molecules, nano-objects) make SAv-biotin technology versatile. SAv-biotin technology is widely used for labeling, detection and purification, enabling a wide range of experimental designs, and also provides the basis for biosensors, biospecific targeting, drug delivery systems, bioactive coatings and various other super Molecular structures lay the foundation.

Our Service

CD BioSciences provides services for the modification of VLPs by noncovalent bonding methods (based on chain affinity-biotinylation technology) to customers worldwide at the most competitive prices.

Of course, our expertise includes but is not limited to noncovalent bonding modification of VLPs, we can also provide you with other chemical conjugation methods (covalent bonding modification) to modify VLPs. CD BioSciences also offers VLPs construction by different plant platforms, VLPs purification and VLPs characterization assays, so please feel free to contact us if you are interested.

VLPlant^TM is Our Core
As a global custom service provider, CD BioSciences has the proven technology, advanced equipment and experienced science to provide you with everything you need to know about building VLPs for your plant platform.

First-class Service
From project consultation to delivery of results, we will stay in touch with you to help you discuss problems encountered in your experiments with our experienced scientists in a timely manner, update solutions in real time, and meet your needs for your project within your budget.

Choose CD BioSciences
No matter where you are in the production of VLPs, we can help, from selecting different plant platforms to VLPs modification and characterization assays, we have the technology and expertise to be your partner of choice.

Reference

1. Hamming PHE.; Huskens J. Streptavidin Coverage on Biotinylated Surfaces. ACS Appl Mater Interfaces. ACS Appl Mater Interfaces. 2021, 48:58114-58123.