Acidic Amino Acids-based VLP-Peptide Conjugation Service
As the world's leading customized service provider, CD BioSciences has a sophisticated VLPlantTM platform, advanced equipment and experienced scientists, dedicated to helping our customers connect peptides and VLPs through acidic amino acids-based modification. Working closely with our customers and experts, we are happy to meet each of our specific requirements in order to provide the optimal solution.
Roles of Peptides in Immunology
Peptides are small natural biomolecules between amino acids and proteins, formed from amino acid monomers linked by peptide (amide) bonds. Many of the roles played by peptides in immunology are primarily determined by epitopes, the most important of which are to serve as immunogenic epitopes to stimulate adaptive immune responses. Epitopes are the smallest chemical molecules that the immune system can recognize, and major histocompatibility complexes (T-cell epitopes) and antibodies (B-cell epitopes) are central to the adaptive immune response. Peptides mediate adaptive immune responses through the interaction of T-cell epitopes with B-cell epitopes.
The stoichiometry of peptide display is defined by the quaternary structure, and the self-assembly of the capsid in vivo requires less processing and a more homogeneous final product. Peptides can be linked to VLPs by genetic fusion or chemical conjugation methods, developing cVLPs. The chemical conjugation methods include cysteine modification, lysine modification, acidic amino acid modification and the like.
VLP-Peptide conjugation. (Servid A, et al., 2013)
- Acidic Amino Acid-based VLP-Peptide Conjugation
Acidic amino acids are amino acids containing two carboxyl groups and one amino group, mainly glutamate and aspartate. Aspartate is used medicinally to treat heart disease, as a liver function enhancer, as a detoxifier, etc. Glutamate contains two carboxyl groups, which are important for protein metabolism in organisms and participate in many important chemical reactions in plants, animals and microorganisms.
Chemical conjugation methods for linking polypeptides to VLPs via aspartate or glutamate modifications differ from those involving cysteine and lysine modifications. Aspartic acid and glutamic acid must be activated with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) because only after activation can their carboxyl groups interact with n-Hydroxysuccinimide (NHS) reacts to form NHS esters. The carboxylic acid side chain can then form stable amide bonds with polypeptides with primary amino acids.
CD BioSciences has the proven VLPlantTM and extensive experience in providing acidic amino acid-based VLP-peptide conjugation. If you are interested in our services, you can contact us directly for more details. We are glad to hear from you and look forward to working with you.
The Workflow of Acidic Amino Acid VLP-Peptide Conjugation
Why Choose CD BioSciences
We provide global customers with services of linking peptides and VLPs based on lysine modification for a long time and are good at solving difficulties and challenges encountered in the experimental process.
We maintain close contact with customers, discuss problems encountered in the experimental process in a timely manner, and meet your specific requirements.
We have the ability and confidence to provide you with the best experimental solution to meet your needs for fast and accurate linking of peptides to VLPs.
- Servid A.; et al. Location of the bacteriophage P22 coat protein C-terminus provides opportunities for the design of capsid-based materials. Biomacromolecules. 2013,14:2989-2995.