Cat No. CP-11411
Product Size
Online Inquiry| Target Information | |
|---|---|
| Product Name | Alpha-Actin Filaments (F-Actin) |
| Species | Rabbit |
| Purification Method | Gel Permeation Chromatography |
| Background | G-Actin prepared from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42 kDa ( 375 amino acids). Actin filaments are obtained by salt induced polymerization of G-Actin. G-Actin from Hypermol is column purified (>99% purity) and pre-centrifuged at 100.000xg, 3h prior to initiation of polymerization. |
| Product Details | |
|---|---|
| Cat. No. | CP-11411 |
| Description | Actin Filaments (Rabbit Skeletal Muscle Alpha Actin) |
| Buffer | 0.1M KCl, 1.0mM ATP, 2.0mM MgCl2. Buffer: Imidazole pH 7.4. |
| Concentration | 2mg/ml |
| Purity | >99% by scanning densitometry |
| Purification Method | Gel Permeation Chromatography |
| Storage | F-Actin is kept on ice and should be used within 2-3 weeks. A fresh 100mM ATP solution (is shipped with the product to add 1.0mM after each week of storage to prevent denaturing of actin. Do not freeze. |
| Disclaimer note | The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
For research use only. Not intended for any clinical use.