Intermediate filament (IF) supergene family proteins are prevalent structural components that form the cytoskeleton in animal tissues in a cell type-specific manner. All IF proteins show a distinctly organized, extended α-helical conformation prone to form two-stranded coiled coils, which are the basic building blocks of these highly flexible, stress-resistant cytoskeletal filaments. CD BioSciences has become a trusted and reliable supplier to many leading international research institutions and clinical diagnostic companies by providing quality products to scientists in the life sciences worldwide. Our antibodies combine specificity, sensitivity and reproducibility to provide high quality antibody reagents for cytoskeleton research.
Molecular structure of the waveform protein tetramer [1].
Different Types of Intermediate Filaments
Based on the amino acid sequence, gene structure, assembly properties, and tissue-specific expression patterns of intermediate filament proteins during development, intermediate filaments can be classified into six major types.
A few common Intermediate Filaments proteins are listed in the following.
| Keratin |
Keratin is one of the family of fibrous structural proteins, which are the main proteins that make up the outer layers of hair, horns, claws and human skin. Keratin protects epithelial tissue cells from damage or stress. Keratin monomers form bundles to form intermediate fibronectin. |
| Vimentin |
Waveform proteins provide elasticity not found in microtubules and actin, and are therefore responsible for maintaining the integrity of the cytoskeleton. |
| Knotin |
Knotin is a type III intermediate filament protein with a molecular weight of 56 kDa found near the Z-line of myosin. Knotin is only expressed in vertebrates, but its homologs are found in many organisms. |
| Syncolin |
Microtubule-associated proteins found in chicken erythrocytes that bind microtubules into bundles. |
| Nestin |
Nestin is a marker molecule of neural stem cells, pancreatic stem cells, and is a member of the intermediate fiber family, which is classified as a class VI intermediate fiber and differs in structure and molecular weight from other intermediate fiber members. |
We offer ready-to-use antibodies for intermediate filaments, please visit our product page if you are interested.
Our Services
CD BioSciences offers a wide range of monoclonal, polyclonal and recombinant antibodies against intermediate filament proteins. We are dedicated to the development and preparation of antibodies for various applications, providing high quality antibody products and R&D services to research institutes and pharmaceutical companies.
- Monoclonal and Polyclonal Antibody Development for Intermediate Filaments
The use of polyclonal and monoclonal antibodies has increased significantly in recent years. Each type of antibody has its own advantages and disadvantages, and we offer efficient antibody production services, so you can choose according to your research needs.
|
Monoclonal Antibodies |
Polyclonal Antibodies |
| Preparation Cost |
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* |
| Preparation Speed |
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| Non-specific Antibodies |
* |
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| Recognition Sites |
Single Table Location |
Multiple Table Locations |
| Batch Differences |
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| Stabilization |
* |
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| Advantages |
Commonly used to detect mixed proteins with high specificity. |
Usually preferred for detection of denatured proteins with higher affinity. |
- Model Organism Intermediate Filament Antibody Development
We develop and produces intermediate filament antibodies for a wide range of model organisms, providing researchers with antibodies to model organism cytoskeletal targets, including human, monkey, murine, rabbit, guinea pig, zebrafish, Drosophila, and C. elegans.
- Intermediate Filament Antibody Development from Different Hosts
Mice and rats are the host species most commonly used to produce research antibodies, especially in the case of monoclonal antibodies. Whereas polyclonal antibodies can be produced in mice or rats, they are usually made in larger animals, such as rabbits, goats, donkeys or sheep, from which it is easier to collect large amounts of serum.
※ There are different subclasses of IgG prepared using different species of animals, and the common subclasses of IgG from different species are as follows.
| Species |
IgG Subclass |
| Rabbit |
IgG (no subclass) |
| Mouse |
IgG1, IgG2a, IgG2b, IgG2c, IgG3 |
| Rat |
IgG1, IgG2a, IgG2b, IgG2c, IgM |
| Goat |
IgG1, IgG2 |
| Caw |
IgG1, IgG2 |
| Horse |
IgGa, IgGb, IgGc, IgG(B), IgG(T) |
| Pig |
IgG1, IgG2a, IgG2b, IgG3, IgG4 |
We provide antibody development services for different hosts (mouse, rat, rabbit, goat, etc.) according to the needs of different customers and are committed to fulfill your experimental needs in all aspects.
Antibody Customization Process
Our Advantages
Advanced Biotechnology
Customizable Designs
Competitive Pricing
Best After-sales Service
CD BioSciences has a professional team and advanced equipment, and the whole process is operated by experienced technicians to provide our customers with cytoskeleton-related research service. If you have any needs, please contact us.
Reference
- Pollard T D, et al. Intermediate Filaments, a central player in cell shape and movement[J]. science, 2009, 326(5957): 1208-1212.
For research use only. Not intended for any clinical use.
