Product Overview
                                                                                                                                                                                                            
                                        
                                            Parental Cell Line 
                                        
                                        
                                            HEK293T                                        
                                     
                                                                                                                                                        
                                                                                                
                                                                                                
                                        
                                            Mutation Description 
                                        
                                        
                                            Knockout achieved by using CRISPR/Cas9, insertion of the selection cassette in exon 7.                                        
                                     
                                                                                                
                                        
                                            Knockout Validation 
                                        
                                        
                                            Sanger Sequencing                                        
                                     
                                                                                                                                                                                                                                                                                                                                                                                                                                        Cell Line Properties
                                                                                                                                                                                                                                        
                                                                                                
                                                                                                                                                                                                                
                                                                                                                                                                                                                                                                                                                                                            
                                                                                                
                                                                                        Target Information
                                                                                                                                                                                                                                                                                                                                                                                                                                            
                                                                                                
                                                                                                
                                                                                                
                                        
                                            Gene Function 
                                        
                                        
                                            Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton.                                        
                                     
                                                                                                
                                        
                                            Sequence Similarities 
                                        
                                        
                                            Belongs to the SCAR/WAVE family. Contains 1 WH2 domain.                                        
                                     
                                                                                                                                                                                                                                                                Storage & Handling
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
                                        
                                            Cryopreservation Cell Medium 
                                        
                                        
                                            Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.                                        
                                     
                                                                                                
                                        
                                            Culture Medium 
                                        
                                        
                                            DMEM (High Glucose) + 10% FBS                                        
                                     
                                                                                                
                                        
                                            Initial Handling Guidelines 
                                        
                                        
                                            Lyophilizate may be stored at 4 °C. After reconstitution, store at -20 °C for short-term storage or -80 °C for long-term storage.                                        
                                     
                                                                                                
                                        
                                            Storage Instructions 
                                        
                                        
                                            Store at -80 °C. Avoid freeze / thaw cycles.