Product Size
                                        
                                        1 x 106 cells/vial, 1 mL                                        
                         
                       
                            Product Overview
                                                                                                                                                                                                            
                                                                                                                                                        
                                                                                                                            
                                                                                                
                                        
                                            Knockout Validation 
                                        
                                        
                                            Sanger Sequencing                                        
                                     
                                                                                                                                                                                                                                                                                                                                                                                                                                                                    Cell Line Properties
                                                                                                                                                                                                                                        
                                        
                                            Tissue 
                                        
                                        
                                            Acute monocytic leukemia                                        
                                     
                                                                                                
                                                                                                                                                                                                                
                                                                                                
                                        
                                            Adherent / Suspension 
                                        
                                        
                                            Suspension                                        
                                     
                                                                                                
                                                                                                                                                                                                                                                                                                                                                                                Target Information
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
                                                                                                
                                                                                                
                                                                                                
                                        
                                            Gene Function 
                                        
                                        
                                            Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.                                        
                                     
                                                                                                
                                        
                                            Sequence Similarities 
                                        
                                        
                                            Contains 1 FERM domain.Contains 1 I/LWEQ domain.                                        
                                     
                                                                                                                                                                                                                                    Storage & Handling
                                                                                                                                                                                                                                                                                                                            
                                        
                                            Recommended Control 
                                        
                                        
                                            Human wild-type THP-1 cell line.                                        
                                     
                                                                                                                                                                                                                                                                                                                                                                                        
                                        
                                            Cryopreservation Cell Medium 
                                        
                                        
                                            Cell freezing medium - DMSO serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.                                        
                                     
                                                                                                
                                        
                                            Culture Medium 
                                        
                                        
                                            RPMI + 10% FBS + 0.05 mM β-mercaptoethanol                                        
                                     
                                                                                                
                                        
                                            Initial Handling Guidelines 
                                        
                                        
                                            Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80 °C. Storage at -80 °C may result in loss of viability.                                        
                                     
                                                                                                
                                        
                                            Storage Instructions 
                                        
                                        
                                            Shipped on dry ice. Store in liquid nitrogen.                                        
                                     
                                                                                                
                                        
                                            Storage Buffer 
                                        
                                        
                                            Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether.